Journal:

Article Title: Retrovirus Vectors Bearing Jaagsiekte Sheep Retrovirus Env Transduce Human Cells by Using a New Receptor Localized to Chromosome 3p21.3

doi:

Figure Lengend Snippet: Host range of LAPSN vector produced by JSRV-pseudotype packaging cells

Article Snippet: Mammalian cells, including SSF-123 primary sheep skin fibroblasts (gift from William Osborne, University of Washington, Seattle), HT-1080 human fibrosarcoma cells (American Type Culture Collection [ATCC] cell line CCL-121), 293 human kidney epithelial cells (ATCC CRL 1573), IB3 immortalized human bronchial epithelial cells ( 62 ), HeLa cervical carcinoma cells (ATCC CCL-2), NIH 3T3 thymidine kinase-deficient mouse embryo fibroblasts ( 60 ), Mus dunni tail fibroblasts ( 11 ), D17 canine osteosarcoma cells (ATCC CRL-6248), 208F rat embryo fibroblasts ( 51 ), MDBK bovine kidney epithelial cells (ATCC CCL-22), Vero African green monkey kidney epithelial cells (ATCC CCL-81), MF-NAN primary mouse (BALB/c) fibroblasts, MF-H1 primary mouse (C57BL/6) fibroblasts, and RbTE rabbit tracheal epithelial cells (gifts from Christine Halbert, Fred Hutchinson Cancer Research Center), were grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Hyclone).

Techniques: Plasmid Preparation, Produced

Journal: Biomolecules

Article Title: Application of Zebrafish Model in the Suppression of Drug-Induced Cardiac Hypertrophy by Traditional Indian Medicine Yogendra Ras

doi: 10.3390/biom10040600

Figure Lengend Snippet: Dose screening and oxidative stress analysis in H9C2 cells exposed to isoproterenol (ISP) and Yogendra Ras (YDR). ( A ) MTT-based dose screening in the H9C2 cells showed a dose-dependent loss of cell viability following exposure to the ISP (IC 20 = 50 µM and IC 50 = 122.4 µM) and YDR (IC 20 = 30 µg/mL and IC 50 = 205 µg/mL). ( B ) Stimulation of the H9C2 cells with ISP (50 µM) induced up-regulation in the production of intracellular reactive oxygen species and its reduction following treatment with YDR. ( C ) Mitochondrial membrane potential (MMP) increment was detected in the ISP-stimulated H9C2 cells. It was reduced following co-treatment with YDR. ( D ) YDR also reduced the ISP-stimulated generation of superoxide ions (O −2 ) in H9C2 cardiomyocytes as indicated through the intracellular formation of formazan in the NBT assay. ( E ) Reduction in the generation of O −2 in the ISP-stimulated H9C2 cells following treatment with YDR was confirmed through the recovery in the intracellular levels of superoxide dismutase (SOD) enzyme. ( F ) Intracellular catalase enzyme levels were also recovered in the ISP-stimulated H9C2 cells following treatment with YDR. All the experiments were performed in biological and technical triplicates. Results represent mean ± SD. The statistical analysis results were analyzed using one-way ANOVA followed by Dunnett’s post-hoc test: p -value # < 0.01 (ISP alone versus normal control) and ** < 0.01, *** < 0.001 (YDR co-treatment versus ISP alone).

Article Snippet: The H9C2 (rat embryonic cardiac) cells were purchased from the ATCC licensed cell repository National Centre for Cell Science (NCCS), Pune, India.

Techniques: Membrane, Control

Journal: Biomolecules

Article Title: Application of Zebrafish Model in the Suppression of Drug-Induced Cardiac Hypertrophy by Traditional Indian Medicine Yogendra Ras

doi: 10.3390/biom10040600

Figure Lengend Snippet: Down-regulation of cardiac-hypertrophy-associated non-clinical and clinical molecular biomarkers by Yogendra Ras (YDR) in the isoproterenol (ISP)-stimulated H9C2 cells. H9C2 cells stimulated with ISP showed an increase in the mRNA expression levels of the pro-inflammatory mediator genes: ( A ) Cyclooxygenase-2 (COX-2), ( B ) NADPH oxidase-2 (NOX-2). and ( C ) NADPH oxidase-4 (NOX-4) using quantitative real-time PCR. Significant down-regulation of these pro-inflammatory genes was observed following a co-treatment of the ISP-stimulated H9C2 cells with YDR. Treatment of the H9C2 cells with ISP also induced an up-regulation of the expression of the fetal cardiac fetal gene ( D ) atrial natriuretic factor (ANF) and ( E ) cardiolipin (CRLS-1), ( F ) troponin I (TNN-I), and ( G ) troponin T (TNN-T). Expression of all the genes was normalized against the house-keeping gene β-actin. Co-treatment of the ISP-stimulated H9C2 cells with YDR significantly reduced the expression of all the up-regulated non-clinical and clinical genes. H) The heat map was generated to summarize the gene expression study. All the experiments were performed in biological and technical triplicates. Results represent mean ± SD. For the statistical analysis, results were analyzed using one-way ANOVA followed by Dunnett’s post-hoc test: p -value # < 0.01 (ISP/YDR versus normal control) and * < 0.05, **< 0.01 (YDR co-treatment versus ISP alone).

Article Snippet: The H9C2 (rat embryonic cardiac) cells were purchased from the ATCC licensed cell repository National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Generated, Gene Expression, Control